Involvement of the mannose receptor in the uptake of der p 1, a major mite allergen, by human dendritic cells

详细信息    查看全文

• 作者：Gaë ; tan Deslé ; e ; Anne-Sophie Charbonnier ; Hamida Hammad ; Gerhild Angyalosi ; Isabelle Tillie-Leblond ; Alberto Mantovani ; Andr é ; -Bernard Tonnel ; Joë ; l Pestel
• 刊名：Journal of Allergy and Clinical Immunology
• 出版年：2002
• 出版时间：November 2002
• 年：2002
• 卷：110
• 期：5
• 页码：763-770
• 全文大小：197 K

文摘

Background: Immature dendritic cells (DCs) take up antigens in peripheral tissues and, after antigen processing, mature to efficiently stimulate T cells in secondary lymph nodes. In allergic airway diseases DCs have been shown to be involved in the induction and maintenance of a T_H2-type profile. Objective: The present study was undertaken to determine pathways of Der p 1 (a house dust mite allergen) uptake by human DCs and to compare Der p 1 uptake between DCs from patients with house dust mite allergy and DCs from healthy donors. Methods: Monocyte- derived DCs (MD-DCs) were obtained from patients with house dust mite allergy (n = 13) and healthy donors (n = 11). Der p 1 was labeled with rhodamine. Der p 1 uptake by MD-DCs was analyzed by means of flow cytometry and confocal microscopy. Results: Rhodamine- labeled Der p 1 was demonstrated to be taken up by MD-DCs in a dose-, time-, and temperature- dependent manner. The involvement of the mannose receptor (MR) in the Der p 1 uptake was demonstrated by using (1) inhibitors of the MR- mediated endocytosis (mannan and blocking anti-MR mAb), which inhibited the Der p 1 uptake from 40 % to 50 % , and (2) confocal microscopy showing the colocalization of rhodamine-labeled Der p 1 with FITC-dextran. Interestingly, compared with DCs from healthy donors, DCs from allergic patients expressed more MR and were more efficient in Der p 1 uptake. Conclusion: These results suggest that the MR could play a key role in the Der p 1 allergen uptake by DCs and in the pathogenesis of allergic diseases in dust mite –sensitive patients. (J Allergy Clin Immunol 2002;110:763-70.)