Mise au point et validation d'une m¨¦thode de dosage de l'amphot¨¦ricine B par chromatographie liquide ¨¤ haute performance

详细信息    查看全文

• 作者：Emna Gaï ; es ; ^{gaiesemna@yahoo.fr} ; Ridha Ben Ali ; Sameh Trabelsi ; Nadia Jebabli ; Issam Salouage ; Rim Charfi ; Mohamed Lakhal ; Anis Klouz
• 关键词：Amphoté ; ricine B ; dosage plasmatique ; CLHP
• 刊名：Revue Francophone des Laboratoires
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：2013
• 期：449_part_P1
• 页码：67-70
• 全文大小：87 K

文摘

| Figures/TablesFigures/Tables | ReferencesReferences

Summary

Amphotericin B is an effective antifungal drug used in the treatment of systemic mycosis, but it has a narrow therapeutic interval and an important renal toxicity. The aim of this study is to monitor and valid a new method for Amphotericin B quantitation in plasma using HPLC with UV/visible detection. A liquid-liquid extraction was done; thereafter, a chromatographic separation was achieved using an RP 18 column (125 mm). The mobile phase is composed of water, acetonitrile and acetic acid (51/44/5) (v/v/v), pH 3. Piroxicam is used as an internal standard. This technique is linear over the range 0,125 ¦Ìg/mL to 8 ¦Ìg/mL (r = 0,99). Detection and quantification limits were 0.044 ¦Ìg/mL and 0.111 ¦Ìg/mL, respectively. Within-day coefficient of variation (1,98 to 4,66 % ) and day-to-day coefficient of variation (2,69 to 4,95 % ) were determined at three different concentrations. Under these conditions, each analysis requires no longer than 10 min.