文摘
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Summary
A high-performance liquid chromatography method was developed for the rapid determination of teicoplanine in human serum. After protein precipitation and extraction, chromatographic separation of teicoplanine in human serum was achieved with a C18 column. The mobile phase consisted of phosphate buffer, methanol and acetonitrile 80/11/9 (v/v/v), pH 4. Quantitative determination was monitored using UV detector at wavelength of 220 nm. Sulfamethoxazole was used as internal standard. Calibration curve is linear over the range 0.5 to 30 ¦Ìg/mL (r = 0.99). Limit of detection and limit of quantification are 0.034 and 0,104 ¦Ìg/ mL, respectively. Within-day coefficient of variation (1.31 to 2.64 % ) and day-to-day coefficient of variation (4.46 to 5.72 % ) at three different concentrations are accurate for this analysis. Relative recovery ranges from 98.14 to 99.4 % at three different concentrations. Each analysis requires no longer than 12 min.
