Oligonucleotide array CGH studies in myeloproliferative neoplasms: Comparison with JAK2
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- 作者：Ayalew Tefferi ; Shireen Sirhan ; Yi Sun ; Terra Lasho ; Christy M. Finke ; James Weisberger ; Sherri Bale ; John Compton ; Charles A. LeDuc ; Animesh Pardanani ; Erik C. Thorl ; Yuriy Shevchenko ; Marc Grodman
- 关键词：Array CGH ; Myeloproliferative ; Polycythemia ; Thrombocythemia ; Myelofibrosis ; JAK2 V617F
- 刊名：Leukemia Research
- 出版年：2009
- 出版时间：May 2009
- 年：2009
- 卷：33
- 期：5
- 页码：662-664
- 全文大小：113 K
文摘
Comparative genomic hybridization (CGH), using oligo arrays with either 44,000 or 105,000 oligonucleotides, was performed on granulocyte-derived DNA from 71 patients with BCR-ABL-negative classic myeloproliferative neoplasms (MPNs): 32 primary myelofibrosis (PMF), 26 polycythemia vera (PV) and 13 essential thrombocythemia (ET). Copy number changes (CNCs) were detected in 44 % , 35 % , and 15 % of the cases with PMF, PV and ET, respectively. In ET and PMF, CNCs were more frequently detected in the presence of JAK2V617F (50 % vs. 19 % ; p = 0.05). Conventional chromosome analysis was obtained in 57 patients either at diagnosis or within 1 year of the array CGH study; all 21 patients with PV and 11 with ET displayed normal cytogenetic findings despite the presence of CNCs in 29 % and 18 % , respectively. In PMF, the respective rates of CNCs and abnormal karyotype were 48 % and 36 % ; karyotypic abnormalities, including unbalanced translocations, were often detected by array CGH as chromosomal gains or losses. This preliminary report suggests a potential value for array CGH in terms of both clinical diagnostics and genomic research in MPNs.