ESI-IMS-MS: A method for rapid analysis of protein aggregation and its inhibition by small molecules
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- 作者：Lydia M. Younga ; b ; 1 ; bs08lw@leeds.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Janet C. Saundersa ; b ; 1 ; bsjct@leeds.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Rachel A. Mahooda ; b ; bs08ram@leeds.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Charlotte H. Revilla ; c ; C.H.Revill@leeds.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Richard J. Fostera ; c ; R.Foster@leeds.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Alison E. Ashcrofta ; b ; A.E.Ashcroft@leeds.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Sheena E. Radforda ; b ; S.E.Radford@leeds.ac.uk" class="auth_mail" title="E-mail the corresponding author
- 关键词：Aβ ; amyloid-β peptide ; Aβ40 ; amyloid-β peptide residues 1&ndash ; 40 ; AD ; Alzheimer&rsquo ; s disease ; ADH ; alcohol dehydrogenase ; AFM ; atomic force microscopy ; CCS ; collision-cross sectional area ; Cl-NQTrp ; chloronaphthoquinine-tryptophan ; CsI ; caesium iodide ; DMSO ; dimethyl sulfoxide ; EGCG ; (&minus ; )-epigallocatechin gallate ; ESI-IMS&ndash ; MS ; electrospray ionisation-ion mobility spectrometry&ndash ; mass spectrometry ; HDMS ; high-definition mass spectrometry ; hIAPP ; human islet amyloid polypeptide
- 刊名：Methods
- 出版年：2016
- 出版时间：15 February 2016
- 年：2016
- 卷：95
- 期：Complete
- 页码：62-69
- 全文大小：2777 K
文摘
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Identification of small molecule inhibitors of protein aggregation using ESI-IMS–MS.
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Characterisation of binding mode as negative, specific, non-specific or colloidal.
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High throughput ligand screening using ESI-IMS–MS.
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Identification of novel small molecule inhibitors of Aβ40 aggregation.