The Tunisian oasis ecosystem is a source of antagonistic Bacillus spp. producing diverse antifungal lipopeptides

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• 作者：Amel El Arbi^a ; ^b ; ^{amel_elarbi@yahoo.fr" class="auth_mail" title="E-mail the corresponding author} ; Alice Rochex^a ; ^{alice.rochex@polytech-lille.fr" class="auth_mail" title="E-mail the corresponding author} ; Gabrielle Chataign&eacute ; ^a ; ^{gabrielle.chataigne@polytech-lille.fr" class="auth_mail" title="E-mail the corresponding author} ; Max B&eacute ; chet^a ; ^{max.bechet@univ-lille1.fr" class="auth_mail" title="E-mail the corresponding author} ; Didier Lecouturier^a ; ^{didier.lecouturier@univ-lille1.fr" class="auth_mail" title="E-mail the corresponding author} ; S&eacute ; golè ; ne Arnauld^a ; ^{segolone.arnauld@polytech-lille.fr" class="auth_mail" title="E-mail the corresponding author} ; N&eacute ; ji Gharsallah^b ; ^{Neji.Gharsallah@fss.rnu.tn" class="auth_mail" title="E-mail the corresponding author} ; Philippe Jacques^a ; ^{philippe.jacques@polytech-lille.fr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Bacillus ; Antifungal lipopeptides ; Biological control ; Date palm rhizosphere ; Oasis ecosystem
• 刊名：Research in Microbiology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：167
• 期：1
• 页码：46-57
• 全文大小：1405 K

文摘

The use of microbial products has become a promising alternative approach to controlling plant diseases caused by phytopathogenic fungi. Bacteria isolated from the date palm tree rhizosphere of the Tunisian oasis ecosystem could provide new biocontrol microorganisms adapted to extreme conditions, such as drought, salinity and high temperature. The aim of this study was to screen bacteria isolated from the rhizosphere of the date palm tree for their ability to inhibit phytopathogenic fungi, and to identify molecules responsible for their antifungal activity. Screening for antifungal activity was performed on twenty-eight isolates. Five antagonistic isolates were selected and identified as different species of Bacillus using phenotypical methods and a molecular approach. The five antagonistic Bacillus isolated showed tolerance to abiotic stresses (high temperature, salinity, drought). Their ability to produce lipopeptides was investigated using a combination of two techniques: PCR amplification and MALDI-ToF mass spectrometry. Analyses revealed that the antagonistic isolates produced a high diversity of lipopeptides that belonged to surfactin, fengycin, iturin and kurstakin families. Their antagonistic activity, related to their capacity for producing diverse antifungal lipopeptides and their tolerance to abiotic stresses, highlighted Bacillus strains isolated from the rhizosphere of the date palm tree as potential biocontrol agents for combatting plant diseases in extreme environments.