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- 作者：Maghazachi ; Azzam A. ; Knudsen ; Eirunn ; Jin ; Yixin ; Jenstad ; Monica ; Chaudhry ; Farrukh A.
- 关键词：NK cells ; Glycosphingolipids ; Lysophospholipids ; Apoptosis ; Multinucleation ; Vacuolation ; Chemotaxis ; Cytotoxicity ; Interferon γ
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2004
- 出版时间：July 30, 2004
- 年：2004
- 卷：320
- 期：3
- 页码：810-815
- 全文大小：482 K
文摘
Natural killer (NK) cells perform multiple biological functions including tumor cell lysis and eradicating virally infected cells. Here, we report for the first time that d-galactosyl-β1-1′ sphingosine and d-glucosyl-β1-1′ sphingosine damage human NK cells. We show that these cells express T-cell-associated gene-8, the receptor for glycosphingolipids. d-Galactosyl-β1-1′ sphingosine and d-glucosyl-β1-1′ sphingosine induce the in vitro chemotaxis of human NK cells. Both d-galactosyl-β1-1′ sphingosine and d-glucosyl-β1-1′ sphingosine inhibit the cytotoxicity and IFN-γ secretion by these cells. Further analysis shows that the glycosphingolipids d-galactosyl-β1-1′ sphingosine and d-glucosyl-β1-1′ sphingosine but not any other lipid examined, which include d-lactosyl-β1-1′ sphingosine, sphingosine 1-phosphate, sphingosine, lysophosphatidic acid, and phosphatidic acid, induce the apoptosis, globoid-like formation, and multinucleation in human NK cells. These results may have important implications on diseases where glycosphingolipids accumulate.