PCR-REA as an important tool for the identification of Cryptococcus neoformans and Cryptococcus gattii from human and veterinary sources

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• 作者：Rossana Aguiar  ; Cordeiro^a ;   ; ^b ;   ; ^{rossanacordeiro@ufc.br} ; Ana Karoline Freire  ; Costa^c ; Raimunda Sâ ; mia Nogueira  ; Brilhante^a ;   ; ^b ; Rita Amanda Chaves  ; de Lima^b ; Dé ; bora de Souza Collares Maia  ; Castelo-Branco^b ; Joyce Fonteles  ; Ribeiro^b ; André ; Jalles  ; Monteiro^d ; Francisco Airton Castro  ; Rocha^e ; José ; Jú ; lio Costa  ; Sidrim^a ;   ; ^b ; Marcos Fá ; bio Gadelha  ; Rocha^a ;   ; ^b ;   ; ^c
• 关键词：Cryptococcus ; Identification ; PCR-REA ; API 20C ; VITEK
• 刊名：Veterinary Microbiology
• 出版年：2011
• 出版时间：29 December 2011
• 年：2011
• 卷：154
• 期：1-2
• 页码：180-184
• 全文大小：188 K

文摘

The extraordinary ability of Cryptococcus species to cause disease has focused the attention of scientists on finding ways to improve their identification methods. In this study, PCR-REA, manual methods (morphological and biochemical characteristics), API 20C and VITEK 2 were used to test identify a total of 30 Cryptococcus spp. from human and veterinary sources. PCR-REA was performed using the capsular region as amplification target followed by restriction with the enzymes AgeI, BsmFI and HpaII. PCR-REA identified the strains as C. neoformans var. grubii (n = 19) and C. gattii (n = 8). There was no significant difference between the API 20C AUX and VITEK 2 when compared to manual methods for the identification of Cryptococcus spp. However, none of these non-manual methods were able to detect C. gattii samples. PCR-REA showed a greater level of concordance with the manual method, besides being faster and more sensitive than the other methods. Therefore, it is indicated for routine identification of Cryptococcus spp. strains.