High-throughput assay comparison and standardization for metal chelating capacity screening: A proposal and application
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- 作者：Jâ ; nio Sousa Santosa ; Vitor Rafael Alvarenga Brizolab ; Daniel Granatoa ; b ; dgranato@uepg.br" class="auth_mail" title="E-mail the corresponding author
- 关键词：Iron binding ; Antioxidant activity ; Method validation ; Phenolic compounds ; Metal chelator ; Principal component analysis
- 刊名：Food Chemistry
- 出版年：2017
- 出版时间：1 January 2017
- 年：2017
- 卷：214
- 期：Complete
- 页码：515-522
- 全文大小：812 K
文摘
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Fe2+ and Cu2+ chelating ability micro assays were standardized.
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Coffees were analyzed for metal chelating ability, FRAP, DPPH and total phenolic content.
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The methods presented a good repeatability and reproducibility (<7% RSD).
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Cu2+ chelating ability was correlated to FRAP, DPPH and total phenolic content.