Electrogenerated chemiluminescence biosensing method for the discrimination of DNA hydroxymethylation and assay of the β-glucosyltransferase activity
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- 作者：Huiping Suna ; Shangxian Maa ; Yan Lia ; yanli@nwu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Honglan Qib ; ; Xiaohui Ninga ; Jianbin Zhenga
- 关键词：Electrogenerated chemiluminescence ; DNA hydroxymethylation ; β-glucosyltransferase ; endonuclease ; Tris(2 ; 2&prime ; -bipyridyl)ruthenium
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 May 2016
- 年：2016
- 卷：79
- 期：Complete
- 页码：92-97
- 全文大小：1421 K
文摘
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An ECL biosensing method for discrimination of DNA hydroxymethylation was developed.
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An ECL biosensing method for assay of the β-GT activity was developed.
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The method is based on the β-GT-MspI interaction system and the quenching of ECL.
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The method is PCR-free and sensitive, achieved a lower DL of 0.04 U/mL β-GT.