“Off-On”switching electrochemiluminescence biosensor for mercury(II) detection based on molecular recognition technology

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• 作者：Lin Cheng^a ; BingGuo Wei^a ; Ling Ling He^a ; Ling Mao^a ; Jie Zhang^a ; JinXiang Ceng^b ; DeRong Kong^a ; ChaDan Chen^a ; HanFeng Cui^a ; Nian Hong^a ; ^{327295625@qq.com} ; Hao Fan^a ; ^{fanhao11@aliyun.com}
• 关键词：Electrochemiluminescence ; Biosensor ; Mercury(II) ; Molecular recognition technology ; Switch
• 刊名：Analytical Biochemistry
• 出版年：2017
• 出版时间：1 February 2017
• 年：2017
• 卷：518
• 期：Complete
• 页码：46-52
• 全文大小：946 K
• 卷排序：518

文摘

A novel “off-On” electrogenerated chemiluminescence (ECL) biosensor has been developed for the detection of mercury(II) based on molecular recognition technology. The ECL mercury(II) biosensor comprises two main parts: an ECL substrate and an ECL intensity switch. The ECL substrate was made by modifying the complex of Ruthenium(II) tris-(bipyridine)(Ru(bpy)32+)/Cyclodextrins-Au nanoparticles(CD-AuNps)/Nafion on the surface of glass carbon electrode (GCE), and the ECL intensity switch is the single hairpin DNA probe designed according to the “molecular recognition” strategy which was functionalized with ferrocene tag at one end and attached to Cyclodextrins (CD) on modified GCE through supramolecular noncovalent interaction. We demonstrated that, in the absence of Hg(II) ion, the probe keeps single hairpin structure and resulted in a quenching of ECL of Ru(bpy)32+. Whereas, in the presence of Hg(II) ion, the probe prefers to form the T-Hg(II)-T complex and lead to an obvious recovery of ECL of Ru(bpy)32+, which provided a sensing platform for the detection of Hg(II) ion. Using this sensing platform, a simple, rapid and selective “off-On” ECL biosensor for the detection of mercury(II) with a detection limit of 0.1 nM has been developed.