An interpenetrating HA/G/CS biomimic hydrogel via Diels-Alder click chemistry for cartilage tissue engineering

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• 作者：Feng Yu^a ; ^b ; Xiaodong Cao^a ; ^c ; ^{caoxd@scut.edu.cn} ; Lei Zeng^a ; ^c ; Qing Zhang^a ; ^b ; Xiaofeng Chen^a ; ^b ; ^c ; ^{chenxf@scut.edu.cn}
• 关键词：Gelatin ; Hyaluronic acid ; Chondroitin sulfate ; Interpenetrating hydrogel ; Diels&ndash ; Alder click chemistry
• 刊名：Carbohydrate Polymers
• 出版年：2013
• 出版时间：14 August, 2013
• 年：2013
• 卷：97
• 期：1
• 页码：188-195
• 全文大小：2044 K

文摘

In order to mimic the natural cartilage extracellular matrix, a novel biological degradable interpenetrating network hydrogel was synthesized from the gelatin (G), hyaluronic acid (HA) and chondroitin sulfate (CS) by Diels-Alder ¡°click¡± chemistry. HA was modified with furylamine and G was modified with furancarboxylic acid respectively. ¹H NMR spectra and elemental analysis showed that the substitution degrees of HA-furan and G-furan were 71.5 % and 44.5 % . Then the hydrogels were finally synthesized by cross-linking furan-modified HA and G derivatives with dimaleimide poly(ethylene glycol) (MAL-PEG-MAL). The mechanical and degradation properties of the hydrogels could be tuned simply through varying the molar ratio between furan and maleimide. Rheological, mechanical and degradation studies demonstrated that the Diels-Alder ¡°click¡± chemistry is an efficient method for preparing high performance biological interpenetrating hydrogels. This biomimic hydrogel with improved mechanical properties could have great potential applications in cartilage tissue engineering.