Bacterial community succession during the enrichment of chemolithoautotrophic arsenite oxidizing bacteria at high arsenic concentrations

详细信息    查看全文

• 作者：Nguyen Ai Le^a ; Akiko Sato^a ; Daisuke Inoue^a ; ^b ; Kazunari Sei^a ; ^b ; Satoshi Soda^a ; Michihiko Ike^a ; ^{ike@see.eng.osaka-u.ac.jp}
• 关键词：arsenic ; bacterial community ; chemolithoautotrophic arsenite oxidizing bacteria ; enrichment ; terminal-restriction fragment length polymorphism
• 刊名：Journal of Environmental Sciences
• 出版年：2012
• 出版时间：December, 2012
• 年：2012
• 卷：24
• 期：12
• 页码：2133-2140
• 全文大小：302 K

文摘

To generate cost-effective technologies for the removal of arsenic from water, we developed an enrichment culture of chemolithoautotrophic arsenite oxidizing bacteria (CAOs) that could effectively oxidize widely ranging concentrations of As(III) to As(V). In addition, we attempted to elucidate the enrichment process and characterize the microbial composition of the enrichment culture. A CAOs enrichment culture capable of stably oxidizing As(III) to As(V) was successfully constructed through repeated batch cultivation for more than 700 days, during which time the initial As(III) concentrations were increased in a stepwise manner from 1 to 10-12 mmol/L. As(III) oxidation activity of the enrichment culture gradually improved, and 10-12 mmol/L As(III) was almost completely oxidized within four days. Terminal restriction fragment length polymorphism analysis showed that the dominant bacteria in the enrichment culture varied drastically during the enrichment process depending on the As(III) concentration. Isolation and characterization of bacteria in the enrichment culture revealed that the presence of multiple CAOs with various As(III) oxidation abilities enabled the culture to adapt to a wide range of As(III) concentrations. The CAOs enrichment culture constructed here may be useful for pretreatment of water from which arsenic is being removed.