Immunohistochemical localization of dopamine D2 receptor in the rat carotid body

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• 作者：Jun Wakai^a ; Anna Takayama^b ; Takuya Yokoyama^b ; Nobuaki Nakamuta^b ; Tatsumi Kusakabe^c ; Yoshio Yamamoto^b ; ^{yyoshio@iwate-u.ac.jp" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Dopamine D2 receptor ; Carotid body ; Glomus cell ; Chemoreceptor ; Rat
• 刊名：Acta Histochemica
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：117
• 期：8
• 页码：784-789
• 全文大小：3555 K

文摘

Dopamine modulates the chemosensitivity of arterial chemoreceptors, and dopamine D2 receptor (D2R) is expected to localize in the glomus cells and/or sensory nerve endings of the carotid body. In the present study, the localization of D2R in the rat carotid body was examined using double immunofluorescence for D2R with various cell markers. D2R immunoreactivity was mainly localized in glomus cells immunoreactive to tyrosine hydroxylase or dopamine β-hydroxylase (DBH), but not in S100B-immunoreactive sustentacular cells. Furthermore, D2R immunoreactivity was observed in petrosal ganglion cells and nerve bundles in the carotid body, but not in the nerve endings with P2X2 immunoreactivity. In the carotid ganglion, a few punctate D2R-immunoreactive products were detected in DBH-immunoreactive nerve cell bodies. These results showed that D2R was mainly distributed in glomus cells, and suggested that D2R plays a role in the inhibitory modulation of chemosensory activity in a paracrine and/or autocrine manner.