TRPV1 channels in the nucleus of the solitary tract mediate thermal prolongation of the LCR in decerebrate piglets

详细信息    查看全文

• 作者：Luxi Xia^a ; Donald Bartlett Jr.^a ; J.C. Leiter ; ^a ; ^{james.c.leiter@dartmouth.EDU}
• 关键词：SIDS ; Laryngeal chemoreflex ; Hyperthermia ; GABA ; TRPV1 channels ; Nucleus of the solitary tract
• 刊名：Respiratory Physiology & Neurobiology
• 出版年：2011
• 出版时间：30 April 2011
• 年：2011
• 卷：176
• 期：1-2
• 页码：21-31
• 全文大小：1104 K

文摘

Elevating body temperature or just the temperature of the dorsal medulla by approximately 2 °C prolongs the laryngeal chemoreflex (LCR) in decerebrate neonatal piglets. We tested the hypothesis that transient receptor potential vanilloid 1 (TRPV1) receptors in the nucleus of the solitary tract (NTS) mediate thermal prolongation of the LCR. We studied the effect of a selective TRPV1 receptor antagonist on thermal prolongation of the LCR, and we tested the effect of a TRPV1 agonist on the duration of the LCR under normothermic conditions. We studied 37 decerebrate neonatal piglets between the ages of post-natal days 4 and 7. The TRPV1 receptor antagonist, 5′-iodoresiniferatoxin (65 μM/L in 100 nL), blocked thermal prolongation of the LCR when injected bilaterally into the region of the NTS. The TRPV1 agonist, resiniferatoxin (0.65–1.0 mM/L in 100 nL), prolonged the LCR after bilateral injection into the NTS even when the body temperature of each piglet was normal. The effect of the TRPV1 agonists could be blocked by treatment with the GABA_A receptor antagonist, bicuculline, whether given intravenously (0.3 mg/kg) or focally injected bilaterally into the NTS (10 mM in 100 nL). We conclude that TRPV1 receptors in the NTS mediate thermal prolongation of the LCR.