Ligation of Fc gamma receptor IIB enhances levels of antiviral cytokine in response to PRRSV infection in vitro

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• 作者：Yina Zhang¹ ; Yonghui Zhou¹ ; Qingyuan Yang ; Chunlong Mu ; Erzhen Duan ; Jing Chen ; Mingfan Yang ; Pingan Xia ; ^{xpa88@163.com} ; Baoan Cui
• 关键词：Fc¦Ã receptor ; Fc¦ÃRIIB ; PRRSV ; Cytokine ; Pig
• 刊名：Veterinary Microbiology
• 出版年：2012
• 出版时间：7 December, 2012
• 年：2012
• 卷：160
• 期：3-4
• 页码：473-480
• 全文大小：891 K

文摘

PRRSV infection ADE facilitates the attachment and internalization of the virus onto its host cells, such as monocytes and macrophages, through Fc receptor-mediated endocytosis. Fc¦ÃRIIB is the only inhibitory receptor with a tyrosine-based inhibitory motif (ITIM) in its cytoplasmic tail, where counters the ¡°ITAM triggered¡± activation signals and down-regulates phagocytosis. However, porcine Fc¦ÃRIIB's role in the antiviral immune response to PRRSV infection has not been studied. In this study, our results indicated that selective activation of porcine Fc¦ÃRIIB in PAM cells up-regulated significantly mRNA levels of IFN-¦Á and TNF-¦Á at any time point post-pretreatment, suggesting that porcine Fc¦ÃRIIB signal can enhance the innate antiviral response of host cells. PRRSV infection assay mediated by Fc¦ÃRIIB indicated that selective activation of porcine Fc¦ÃRIIB in PAM cells enhanced mRNA levels of antiviral cytokine (IFN-¦Á and TNF-¦Á) and repressed mRNA levels of IL-10 in response to PRRSV infection, suggesting that Fc¦ÃRIIB ligation can enhance the antiviral immune response to PRRSV infection. In addition, Fc¦ÃRIIB ligation to infection indicated that PRRSV replication in PAM was not positive correlation with increasing of IFN-¦Á mRNA levels and decreasing of IL-10 mRNA levels, suggesting that there is complex viral replication mechanism in immune cells such as PAM for PRRSV.