Recognition of molecularly imprinted polymers for a quaternary alkaloid of berberine

详细信息    查看全文

• 作者：Chia-Yun Chen^a ; Chih-Hung Wang^b ; Arh-Hwang Chen^b ; ^{chenah@ms12.hinet.net"" rel=""nofollow}
• 关键词：Molecularly imprinted polymer ; Quaternary alkaloid ; Molecular recognition ; Adsorption equilibrium ; Solid-phase extraction
• 刊名：Talanta
• 出版年：2011
• 出版时间：30 May 2011
• 年：2011
• 卷：84
• 期：4
• 页码：1038-1046
• 全文大小：780 K

文摘

Selective and affinitive imprinted polymers incorporating a quaternary alkaloid of berberine (BER) were prepared using a non-covalent imprinting method. The results showed that, compared to other imprinted polymers, the polymer AD-10 had not only a higher of the ratio of Q_MIP/Q_BP for BER adsorption, and but also a larger of the ratio of Q_MIP,B/Q_MIP,P for BER and palmatine (PAL) adsorptions. Spectrophotometric analysis demonstrated that a 1:1 cooperative hydrogen-bonding complex might be predominating in the pre-polymerization between the BER template and AA monomer. Adsorption experiments of BER on the polymer AD-10 were in accordance with the second-order and Langmuir adsorption models. The E value (5.70 kJ/mol) calculated from the Dubinin–Radushkevich model indicated that the adsorption followed a physisorption process. In addition, a Scatchard plot showed a single straight line with an equilibrium dissociation constant (K_D) of 65.80 μmol/L. SPE analyses of a mixture of BER and PAL and the methanol extract from the cortices of Phellodendron wilsonii showed that AD-10 had more efficiency, and higher specificity and selectivity for SPE in the concentration and determination of BER and its extraction from natural products.