MiR-572 prompted cell proliferation of human ovarian cancer cells by suppressing PPP2R2C expression

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• 作者：Ai-Hua Wu^a ; ^c ; ¹ ; Yu-ling Huang^b ; ^c ; ¹ ; Lan-Zhen Zhang^a ; Geng Tian^a ; Qiong-Zhi Liao^b ; Shi-Ling Chen^c ; ^{shilingchendr@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：miR-572 ; Ovarian cancer ; PPP2R2C ; Cell proliferation
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：77
• 期：Complete
• 页码：92-97
• 全文大小：1504 K

文摘

Ovarian cancer (OC) remains one of the most common types of malignant cancer, and the molecular mechanism underlying its proliferation is still largely unclear. It is reported that microRNAs acted as important regulators of cell proliferation by regulating its targeted gene. In this study, our result showed that miR-572 was markedly upregulated in OC cell lines and clinical tissues. Results of both gain-of-function and loss-of-function experiments revealed that upregulation of miR-572 expression dramatically promoted OC cell proliferation, whereas decreased miR-572 expression significantly reduced cell proliferation. Bioinformatics analysis and luciferase reporter assays further revealed PPP2R2C, a putative tumor suppressor as a potential target of miR-572. Moreover, silencing of PPP2R2C using small interfering RNA (siRNA) counteracted the proliferation arrest by miR-572-in in OC cells. In sum, our data provide that miR-572 promoted cell proliferation in OC by targeting PPP2R2C and might serve as a therapeutic target of OC.