Attenuation of Isoflurane Preconditioning-Induced Acute Cardioprotection in Hypertensive Hypertrophied Hearts

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• 作者：Chen-Hsiu Chen ; MD ; PhD^* ; ^&Dagger ; ; Chih-Wei Wu ; PhD^* ; Cheng-Dean Shih ; PhD^&dagger ; ; Wei-Hung Lien ; MD^* ; Shiao-Lin Huang ; MD^* ; Cheng-Cheng Huang ; MD^* ; ^{kent7892002@msn.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：hypertension ; ventricular hypertrophy ; preconditioning ; volatile anesthetic
• 刊名：Journal of Cardiothoracic and Vascular Anesthesia
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：30
• 期：5
• 页码：1317-1323
• 全文大小：568 K

文摘

To evaluate the efficiency of isoflurane-induced anesthetic preconditioning and the role of mitochondrial manganese superoxide dismutase (MnSOD) in hypertensive hypertrophied hearts.

Design

A prospective animal investigation.

Setting

Medical center hospital research laboratory.

Participants

Male spontaneously hypertensive rats (SHRs) and normotensive control Wistar-Kyoto (WKY) rats.

Interventions

All pentobarbital-anesthetized open-chest rats were subjected to a 45-minute left coronary artery occlusion followed by a 120-minute reperfusion. Before ischemia, both SHR and WKY rats were assigned randomly to receive a 30-minute exposure to 0.9% saline or 1.0 minimum alveolar concentration isoflurane.

Measurements and Main Results

The myocardial infarct size, assessed as a percentage of the area at risk, was significantly greater in the hypertrophied SHRs than in the WKY rats (65.3%±8.7% v 51.8%±7.2%, p<0.05). Isoflurane preconditioning appreciably reduced the infarct size in the WKY hearts (30.9%±10.5%, p<0.05) but not in the SHR hearts. MnSOD protein expression and enzymatic activity were increased drastically in response to isoflurane exposure in the hearts of the WKY rats (p<0.05) but not in the SHRs.

Conclusions

Isoflurane-induced anesthetic preconditioning is attenuated in hypertensive hypertrophied hearts. This impairment may be associated with the loss of MnSOD augmentation during ischemia and reperfusion.