Anti-metastatic activities of Antrodia camphorata against human breast cancer cells mediated through suppression of the MAPK signaling pathway

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• 作者：Hsin-Ling Yang ; Yueh-Hsiung Kuo ; Ching-Tsan Tsai ; Yi-Ting Huang ; Ssu-Ching Chen ; Hsueh-Wei Chang ; Elong Lin ; Wen-Hsin Lin ; You-Cheng Hseu
• 关键词：Antrodia camphorata ; Breast cancer ; Metastasis ; Matrix metalloproteinase ; Nuclear factor κ ; B ; Chinese herb
• 刊名：Food and Chemical Toxicology
• 出版年：2011
• 出版时间：January 2011
• 年：2011
• 卷：49
• 期：1
• 页码：290-298
• 全文大小：915 K

文摘

The fermented culture broth of Antrodia camphorata (A. camphorata) has been shown to promote cell cycle arrest and apoptosis of human estrogen-nonresponsive MDA-MB-231 cells. Herein, we demonstrate that non-cytotoxic concentrations (20–80 μg/mL) of A. camphorata markedly inhibited the invasion/migration of highly metastatic MDA-MB-231 cells as shown by an in vitro transwell and a wound-healing repair assay. The results of a gelatin zymography assay showed that A. camphorata suppressed the activity of matrix metalloproteinase (MMP)-9 and urokinase plasminogen activator (uPA). Western blot results demonstrated that treatment with A. camphorata decreased the expression of MMP-9, MMP-2, uPA, uPA receptor (uPAR) and vascular endothelial growth factor (VEGF); while the expression of the endogenous inhibitors of these proteins, i.e., tissue inhibitors of MMP (TIMP-1 and TIMP-2), and plasminogen activator inhibitor (PAI)-1, increased. Further investigation revealed that A. camphorata suppressed the phosphorylation of ERK1/2, p38, and JNK1/2. A. camphorata treatment also led to a dose-dependent inhibition on NF-κB binding and activation. This is the first report confirming the anti-metastatic activity of this potentially beneficial mushroom against human breast cancer.