Investigating the relationship between temperature, conformation and calcium binding in heparin model oligosaccharides

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• 作者：Ashley Hughes^a ; ^f ; ¹ ; Maria Meneghetti^b ; ¹ ; Teng-Yi Huang^c ; Shang-Cheng Hung^c ; Stefano Elli^d ; Marco Guerrini^d ; Timothy Rudd^e ; Marcelo Lima^b ; ^f ; ² ; ^{mlima@unifesp.br} ; Edwin Yates^f ; ^b ; ² ; ^{eayates@liv.ac.uk}
• 关键词：13C ; 15N oligosaccharide ; Octasaccharide ; Hydrogen bonding ; Heparin ; Heparan sulfate ; NMR
• 刊名：Carbohydrate Research
• 出版年：2017
• 出版时间：13 January 2017
• 年：2017
• 卷：438
• 期：Complete
• 页码：58-64
• 全文大小：1044 K
• 卷排序：438

文摘

13C/15N labelled heparin octasasccharide was studied by 1H, 13C & 15N NMR. Complex chemical shift changes were observed from 280 to 305 K. A new thermally-sensitive interaction was identified. Ca binding to heparin was maintained regardless of conformational state.