Accurate and easy-to-use assessment of contiguous DNA methylation sites based on proportion competitive quantitative-PCR and lateral flow nucleic acid biosensor
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- 作者：Wentao Xua ; c ; 1 ; Nan Chenga ; 1 ; Kunlun Huanga ; c ; Yuehe Linb ; Chenguang Wanga ; Yuancong Xua ; Longjiao Zhua ; Dan Dub ; Yunbo Luoa ; lyb@cau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：DNA methylation ; Proportion competitive quantitative PCR ; Lateral flow nucleic acid biosensor
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 June 2016
- 年：2016
- 卷：80
- 期：Complete
- 页码：654-660
- 全文大小：1553 K
文摘
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The first application of PCQ-PCR-LFNAB cascade in the detection of the DNA methylation level.
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Accurate detection due to the proportion competitive amplification and the excellent self-correcting capability.
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Easy-to-use assay owning self-contained proportion quantitative system are independent of external standard.