Overexpression of miR-29b reduces collagen biosynthesis by inhibiting heat shock protein 47 during skin wound healing

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• 作者：Yumin Zhu^a ; Zhongwu Li^b ; Yanling Wang^a ; Lin Li^c ; Dongmiao Wang^b ; Wei Zhang^d ; Laikui Liu^a ; ^d ; Hongbing Jiang^a ; ^b ; Jianrong Yang^b ; ^{jianrong_yangnj@163.com" class="auth_mail" title="E-mail the corresponding author} ; Jie Cheng^a ; ^b ; ^{leonardo_cheng@163.com" class="auth_mail" title="E-mail the corresponding author} ; ^{jianrong_yangnj@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：ECM ; extracellular matrix ; HSP47 ; heat shock protein 47 ; miR-29 ; microRNA-29 ; 3&prime ; -UTR ; 3&prime ; untranslated region ; IL-1β ; interleukin-1 beta ; TNF-α ; tumor necrosis factor-alpha ; TGF-β ; transforming growth factor-beta ; PDGF ; platelet-derived growth factor ; immunohistochemical staining ; IHC ; immunofluorescence ; IF ; VEGF ; vascular endothelial growth factor
• 刊名：Translational Research
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：178
• 期：Complete
• 页码：38-53.e6
• 全文大小：5452 K

文摘

Skin scar formation is characterized by excessive synthesis and aberrant deposition of collagens during wound healing. MicroRNAs are endogenous gene regulators critically involved in diverse biological events including skin scar formation and hold considerable promise as therapeutic targets. However, the detailed molecular mechanisms responsible for collagen production during skin wound repair and scar formation remain incompletely known. Here our data revealed that significant downregulation of miR-29b and upregulation of heat shock protein 47 (HSP47) were observed during wound healing in both excisional and burn wound models and also detected in facial skin scar as compared to adjacent healthy skin. HSP47, a specific chaperon for collagen production and secretion, was identified as a novel and direct post-transcriptional target of miR-29b in skin fibroblasts via bioinformatics prediction and experimental validation. Moreover, the regulatory functions of miR-29b in collagen biosynthesis are partially achieved through modulating HSP47 expression in skin fibroblasts. Furthermore, the profibrotic growth factor TGF-β1 inhibited miR-29b transcription by activating TGF-β/Smads signaling and in turn depressed HSP47 and enhanced collagen 1 production. In contrast, the proinflammatory cytokines IL-1β and TNF-α significantly induced miR-29b transcription via activating NF-κB signaling but had no significant effect on HSP47 and collagen production in skin fibroblasts. Importantly, local delivery of miR-29b lentiviral particles inhibited HSP47 expression and collagen biosynthesis as well as suppressed angiogenesis, thus reducing scar formation in an excisional wound splinting model. Collectively, our data reveal that miR-29b can reduce collagen biosynthesis during skin wound healing likely via post-transcriptional inhibition of HSP47 expression. These findings also suggest that therapeutic targeting of miR-29b/HSP47 might be a viable alternative strategy to prevent or reduce scar formation.