Identification of microRNAs in rice root in response to nitrate and ammonium

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• 作者：Hua Li^a ; ^b ; Bin Hu^a ; Wei Wang^a ; Zhihua Zhang^a ; ^b ; Yan Liang^a ; ^b ; Xiaokai Gao^c ; Peng Li^c ; Yongqiang Liu^a ; ^b ; Lianhe Zhang^c ; Chengcai Chu^a ; ^{ccchu@genetics.ac.cn}
• 关键词：Rice ; MicroRNA ; Nitrogen ; Nitrate ; Ammonium
• 刊名：Journal of Genetics and Genomics
• 出版年：2016
• 出版时间：20 November 2016
• 年：2016
• 卷：43
• 期：11
• 页码：651-661
• 全文大小：1825 K
• 卷排序：43

文摘

Nitrate and ammonium are two major nitrogen (N) sources for higher plants, but they differ in utilization and signaling. MicroRNAs (miRNAs) play an essential role in N signal transduction; however, knowledge remains limited about the regulatory role of miRNAs responsive to different N sources, especially in crop plants. To get global overview on miRNAs involved in N response in rice, we performed high-throughput small RNA-sequencing under different nitrate and ammonium treatments. The results demonstrated that only 16 and 11 miRNAs were significantly induced by nitrate and ammonium under short-term treatment, respectively. However, 60 differentially expressed miRNAs were found between nitrate and ammonium under long-term cultivation. These results suggested that miRNA response greatly differentiates between nitrate and ammonium treatments. Furthermore, 44 miRNAs were found to be differentially expressed between high- and low-N conditions. Our study reveals comprehensive expression profiling of miRNAs responsive to different N sources and different N treatments, which advances our understanding on the regulation of different N signaling and homeostasis mediated by miRNAs.