Identification of integrin α1 as an interacting protein of protein tyrosine phosphatase PRL-3

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• 作者：Lirong Peng ; Genglin Jin ; Li Wang ; Jianpin Guo ; Lin Meng and Chengchao Shou
• 关键词：Protein tyrosine phosphatase PRL-3 ; Integrin ; Interaction ; Erk1/2 ; Phosphorylation
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2006
• 出版时间：31 March 2006
• 年：2006
• 卷：342
• 期：1
• 页码：179-183
• 全文大小：324 K

文摘

PRL-3 is a newly identified protein tyrosine phosphatase associated with tumor metastasis. It is over-expressed in various cancers, such as colorectal cancer, gastric cancer, and ovarian cancer, and is correlated with the progression and survival of cancers. Although PRL-3 plays a causative role in promoting cancer cell invasion and metastasis, the molecular mechanism is unknown. To investigate PRL-3’s roles in tumorigenesis and signal transduction pathway, we screened the human placenta brain cDNA library with the bait of PRL-3 in yeast two-hybrid system. Then we identified integrin α1 as a PRL-3-interacting protein for the first time, and verified this physical association with pull-down and co-immunoprecipitation assays. Furthermore, we found that PRL-3 could down-regulate the tyrosine-phosphorylation level of integrin β1 and increased the phosphorylation level of Erk1/2. Our present discovery will provide new clues for elucidating the molecular mechanism of PRL-3 in promoting cancer invasion and metastasis.