The Treatment-Naive Microbiome in New-Onset Crohn鈥檚 Disease

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• 作者：Dirk Gevers¹ ; Subra Kugathasan⁴ ; ²⁴ ; Lee A. Denson⁵ ; ²⁴ ; Yoshiki Vá ; zquez-Baeza⁶ ; Will Van Treuren⁷ ; Boyu Ren⁸ ; Emma Schwager⁸ ; Dan Knights⁹ ; ¹⁰ ; Se Jin Song⁷ ; Moran Yassour¹ ; Xochitl C. Morgan⁸ ; Aleksandar D. Kostic¹ ; Chengwei Luo¹ ; Antonio Gonzá ; lez⁷ ; Daniel McDonald⁷ ; Yael Haberman⁵ ; Thomas Walters¹¹ ; Susan Baker¹² ; Joel Rosh¹³ ; Michael Stephens¹⁴ ; Melvin Heyman¹⁵ ; James Markowitz¹⁶ ; Robert Baldassano¹⁷ ; Anne Griffiths¹⁸ ; Francisco Sylvester¹⁹ ; David Mack²⁰ ; Sandra Kim²¹ ; Wallace Crandall²¹ ; Jeffrey Hyams¹⁹ ; Curtis Huttenhower¹ ; ⁸ ; Rob Knight⁷ ; ²² ; ²³ ; Ramnik J. Xavier¹ ; ² ; ³ ; ^{xavier@molbio.mgh.harvard.edu" class="auth_mail}
• 刊名：Cell Host Microbe
• 出版年：12 March, 2014
• 年：2014
• 卷：15
• 期：3
• 页码：382-392
• 全文大小：2698 K

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Summary

Inflammatory bowel diseases (IBDs), including Crohn鈥檚 disease (CD), are genetically linked to host pathways that implicate an underlying role for aberrant immune responses to intestinal microbiota. However, patterns of gut microbiome dysbiosis in IBD patients are inconsistent among published studies. Using samples from multiple gastrointestinal locations collected prior to treatment in new-onset cases, we studied the microbiome in the largest pediatric CD cohort to date. An axis defined by an increased abundance in bacteria which include聽Enterobacteriaceae, Pasteurellacaea, Veillonellaceae, and Fusobacteriaceae, and decreased abundance in Erysipelotrichales, Bacteroidales, and Clostridiales, correlates strongly with disease status. Microbiome comparison between CD patients with and without antibiotic exposure indicates that antibiotic use amplifies the microbial dysbiosis associated with CD. Comparing the microbial signatures between the ileum, the rectum, and fecal samples indicates that at this early stage of disease, assessing the rectal mucosal-associated microbiome offers unique potential for convenient and early diagnosis of CD.