Sensitive and versatile electrogenerated chemiluminescence biosensing platform for protein kinase based on Ru(bpy)32+ functionalized gold nanoparticles mediated signal transduction
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- 作者：Manman Dong ; Xia Liu ; Qian Dang ; Honglan Qi ; honglanqi@snnu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Yin Huang ; Qiang Gao ; Chengxiao Zhang ; cxzhang@snnu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Electrogenerated chemiluminescence ; Peptide ; Casein kinase II ; Protein kinase A ; Gold nanoparticles
- 刊名：Analytica Chimica Acta
- 出版年：2016
- 出版时间：4 February 2016
- 年：2016
- 卷：906
- 期：Complete
- 页码：72-79
- 全文大小：1392 K
文摘
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A sensitive and versatile ECL biosensing platform is developed for monitoring protein kinase activity and inhibition.
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Ru(bpy)32+ functionalized gold nanoparticles are used as thiol-versatile signal probe.
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The strategy exhibits unique advantages of high sensitivity, good selectivity and versatility.
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The strategy is promising for multiple protein kinase assay and kinase inhibitor profiling.