Formation and characterization of iron-binding phosphorylated human-like collagen as a potential iron supplement

详细信息    查看全文

• 作者：Jianjun Deng ; Fei Chen ; Daidi Fan ; Chenhui Zhu ; Xiaoxuan Ma ; Wenjiao Xue
• 关键词：HLC ; human-like collagen ; G6P ; glucose-6-phosphate ; MOPS ; 3-(N-morpholino)propanesulfonic acid ; MES ; 2-(N-morpholino)ethanesulfonic acid hydrate ; Ferrozine ; 3-(2-pyridyl)-5 ; 6-bis(4-phenylsulfonic acid)-1 ; 2 ; 4-triazine ; ICP ; inductively coupled plasma spect
• 刊名：Materials Science and Engineering: C
• 出版年：2013
• 出版时间：October, 2013
• 年：2013
• 卷：33
• 期：7
• 页码：4361-4368
• 全文大小：803 K

文摘

Iron incorporated into food can induce precipitation and unwanted interaction with other components in food. Iron-binding proteins represent a possibility to avoid these problems and other side effects, as the iron is protected. However, there are several technical problems associated with protein-iron complex formation. In this paper, the iron-binding phosphorylated human-like collagen (Fe-G6P-HLC) was prepared under physiological conditions through phosphorylated modification. One molecule of Fe-G6P-HLC possesses about 24 atoms of Fe. Spectroscopy analysis, differential scanning calorimetry (DSC) and equilibrium dialysis techniques were employed to investigate the characteristics of the Fe-G6P-HLC. The binding sites (n_b) and apparent association constant (K_app) between iron and phosphorylated HLC were measured at n_b = 23.7 and log K_app = 4.57, respectively. The amount of iron (Fe^2 + sulfate) binding to phosphorylated HLC was found to be a function of pH and phosphate content. In addition, the solubility and thermal stability of HLC were not significantly affected. The results should facilitate the utilization of HLC as a bioactive iron supplement in the food and medical industry and provide an important theoretical evidence for the application of HLC chelates.