Improved Genome Editing Efficiency and Flexibility Using Modified Oligonucleotides with TALEN and CRISPR-Cas9 Nucleases
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- 作者：Jean-Baptiste Renaud1 ; Charlotte Boix1 ; Marine Charpentier1 ; Anne De Cian1 ; Julien Cochennec1 ; Evelyne Duvernois-Berthet1 ; Loï ; c Perrouault1 ; Laurent Tesson2 ; 3 ; Joanne Edouard5 ; Reynald Thinard2 ; 3 ; Yacine Cherifi4 ; Sé ; verine Menoret2 ; 3 ; Sandra Fontaniè ; re4 ; Noé ; mie de Crozé ; 5 ; Alexandre Fraichard4 ; Fré ; dé ; ric Sohm5 ; Ignacio Anegon2 ; 3 ; Jean-Paul Concordet1 ; jpconcordet@mnhn.fr" class="auth_mail" title="E-mail the corresponding author ; Carine Giovannangeli1 ; carine.giovannangeli@mnhn.fr" class="auth_mail" title="E-mail the corresponding author
- 刊名：Cell Reports
- 出版年：2016
- 出版时间：8 March 2016
- 年：2016
- 卷：14
- 期：9
- 页码：2263-2272
- 全文大小：2346 K
文摘
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More-efficient genome editing with chemically modified oligonucleotide donors
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Greater flexibility with modified donors, allowing insertions more than 100 bp long
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Efficient mutant isolation in U2OS and RPE1 cell lines, as well as in rat and mouse
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Homozygous loxP site insertion at the mouse ROSA locus