Ultrasensitive enzyme-free electrochemical immunoassay for free thyroxine based on three dimensionally ordered macroporous chitosan-Au nanoparticles hybrid film
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- 作者：Qi Zhanga ; Xiaojun Chena ; chenxj_njut@126.com" class="auth_mail ; Fulai Tub ; Cheng Yaoa ; yaocheng@njtech.edu.cn" class="auth_mail
- 关键词：Three dimensionally ordered macroporous materials ; Chitosan&ndash ; Au nanoparticles hybrid film ; Magnetic multiwall carbon nanotubes ; Free thyroxine ; Sandwich-type electrochemical immunosensor
- 刊名：Biosensors and Bioelectronics
- 出版年：15 September 2014
- 年：2014
- 卷：59
- 期：Complete
- 页码：377-383
- 全文大小：1300 K
文摘
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Free thyroxine concentration in serum is an essential indicator of thyroid function.
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An enzyme-free sandwich electrochemical immunosensor for FT4 was fabricated.
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3D ordered porous chitosan–Au nanoparticles modified electrode was first prepared.
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Magnetic multiwall carbon nanotubes were used as label of secondary antibody.
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The FT4 immunosensor could be applied in clinical assay due to good selectivity.