Characterization of the interaction between Escherichia coli topoisomerase IV E subunit and an ATP competitive inhibitor
详细信息 查看全文
- 作者：Yan Li ; Ying Lei Wong ; Fui Mee Ng ; Boping Liu ; Yun Xuan Wong ; Zhi Ying Poh ; Siew Wen Then ; Michelle Yueqi Lee ; Hui Qi Ng ; Alvin W. Hung ; Joseph Cherian ; Jeffrey Hill ; Thomas H. Keller ; CongBao Kang ; cbkang@etc.a-star.edu.sg" class="auth_mail" title="E-mail the corresponding author
- 关键词：Antibacterial agents ; 19F NMR ; Drug design ; Docking ; Topoisomerase
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2015
- 出版时间：27 November 2015
- 年：2015
- 卷：467
- 期：4
- 页码：961-966
- 全文大小：1516 K
文摘
- •
An N-terminal 24 kDa region of ParE from Escherichia coli (eParE) was obtained.
- •
A bis-pyridylurea inhibitor exhibits an IC50 of 705 nM against eParE.
- •
1H and 19F NMR confirmed eParE and inhibitor interactions.
- •
Inhibitor binds to ATP binding site.