Nano-liquid chromatography in nutraceutical analysis: Determination of polyphenols in bee pollen
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- 作者：Chiara Fanali ; Laura Dugo ; Anna Rocco
- 关键词：Polyphenols ; Nano-liquid chromatography ; Core&ndash ; shell stationary phase ; Bee pollen
- 刊名：Journal of Chromatography A
- 出版年：2013
- 出版时间：25 October, 2013
- 年：2013
- 卷：1313
- 期：Complete
- 页码：270-274
- 全文大小：570 K
文摘
In this study, a nano-liquid chromatography based method for the simultaneous separation of 16 polyphenols employing UV-vis detection has been developed. A 100 ¦Ìm I.D. capillary column packed with C18 core-shell particles (2.6 ¦Ìm particle size, 100 ?) for 10 cm was employed. The separation of analytes was performed with a step gradient in less than 20 min, using 0.5 % formic acid aqueous solution and acetonitrile as eluents. The optimized analytical method was validated and the resulting RSD % for intra-day and inter-day repeatability, related to retention time, retention factor and peak area, were below 4.68 and 5.57 % , respectively. LOD and LOQ values were as low as 0.78 and 3.12 ¦Ìg/mL, while linearity, assessed in the concentration range of interest for all analytes, gave R2 ¡Ý 0.990. The method was finally applied to the analysis of polyphenols extracted from a collected bee pollen. Nine polyphenols, namely o-, p-coumaric acid, ferulic acid, myricetin, cinnamic acid, quercetin, naringenin, hesperitin and kaempferol, were identified. All analytes, with the exception of p-coumaric acid and myricetin, which partially co-eluted with other pollen components, were also quantified in the sample.