Mammalian cell transient expression, non-affinity purification, and characterization of human recombinant IGFBP7, an IGF-1 targeting therapeutic protein
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- 作者：Man-Shiow Jianga ; 1 ; Xiaoyi Yanga ; 1 ; Dominic Espositob ; Earl Nelsona ; Jinhui Yuana ; Ralph F. Hopkinsb ; Trevor Broadta ; Zhen Xiaoc ; Simona Colantonioc ; DaRue A. Prietoc ; Anthony R. Welchd ; Stephen P. Creekmored ; George Mitraa ; Jianwei Zhua ; jianweiz@sjtu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：BRAF ; B-Raf ; CAG ; chicken β-actin ; CHO ; Chinese hamster ovary ; CV ; column volume ; EBV ; Epstein&ndash ; Barr virus ; Fer L ; ferritin composite light chain ; GFP ; green fluorescent protein ; Grp 78 ; glucose-regulated protein 78 ; HEK 293E cell ; human embryonic kidney 293E cells ; HPLC ; high performance liquid chromatography ; HAS ; human serum albumin ; IEF ; isoelectric focusing ; IEX ; ion-exchange ; IGF-1 ; insulin-like growth factor 1 ; IgK ; immunoglobulin kappa chain ; MW ; molecular weight ; PBV ; packed bed vo
- 刊名：International Immunopharmacology
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：29
- 期：2
- 页码：476-487
- 全文大小：1278 K
文摘
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Successfully expressed and purified the mammalian rhIGFBP7, an IGF-1 targeted inhibition protein, using transient gene expression and conventional non-affinity column chromatography purification method.
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High quality material IGFBP7 was generated for further pre-clinical drug development studies.
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Transient gene expression technology through optimization of vectors is practical to be used for production of recombinant non-antibody protein products for biopharmaceutical development.