Immune responses to in ovo vaccine formulations containing inactivated fowl adenovirus 8b with poly[di(sodium carboxylatoethylphenoxy)]phosphazene (PCEP) and avian beta defensin as adjuvants in chickens

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• 作者：Mishal Sarfraz^a ; Muhammed Suleman^b ; Suresh K. Tikoo^a ; ^c ; Colette Wheler^a ; Andrew A. Potter^a ; Volker Gerdts^a ; Arshud Dar^a ; ^{Arshud.Dar@usask.ca}
• 关键词：Fowl adenovirus serotype 8b ; Inclusion body hepatitis ; Broilers ; In ovo vaccines ; Adjuvants ; PCEP ; Avian beta defensin ; Humoral immunity ; Cellular immunity
• 刊名：Vaccine
• 出版年：2017
• 出版时间：7 February 2017
• 年：2017
• 卷：35
• 期：6
• 页码：981-986
• 全文大小：874 K
• 卷排序：35

文摘

Inclusion body hepatitis (IBH) is one of the major viral infections causing substantial economic loss to the global poultry industry. The disease is characterized by a sudden onset of mortality (2–30%) and high morbidity (60–70%). IBH is caused by a number of serotypes of fowl adenovirus with substantially low levels of serotype cross protection. Thus far, there is no effective and safe vaccine commercially available in the North America for the control of IBH in chickens.Poly[di(sodium carboxylatoethylphenoxy)]phosphazene (PCEP) is a high molecular weight, biodegradable water soluble polymer that has been well characterized as a safe and effective adjuvant for a number of experimental veterinary vaccines. Similarly, host defence peptides, including β-defensins, have also been shown to exhibit strong adjuvant potential.In this study, we evaluated the adjuvant activity of PCEP and avian beta defensin (ABD) in a vaccine formulation containing inactivated fowl adenovirus (FAdV) serotype 8b administered in ovo.Our data showed that a combination of PCEP and inactivated virus is capable of inducing a robust and long lasting antibody response. Moreover, significant enhancement of IFN-γ, IFN-α, IL-12(p40) and IL-6 gene expression under the influence of PCEP suggests that as an in ovo adjuvant PCEP has the ability to activate a substantial balanced immune response in chickens. To our knowledge, these are the first studies in which PCEP and ABD have been characterized as adjuvants for the development of an in ovo poultry vaccine. It is expected that these preliminary studies will be helpful in the development of safer and more effective in ovo vaccine against IBH and other infectious diseases affecting chickens.