Detecting trypsin at liquid crystal/aqueous interface by using surface-immobilized bovine serum albumin
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- 作者：Cheng-Hao Chuanga ; Yi-Cheng Linb ; Wei-Long Chenb ; Yu-Hsuan Chenb ; Yu-Xun Chena ; Chieh-Ming Chena ; Hung Wei Shiuc ; Lo-Yueh Changc ; Chia-Hao Chenc ; Chih-Hsin Chenb ; chc@mail.tku.edu.tw" class="auth_mail" title="E-mail the corresponding author
- 关键词：Liquid crystal-based biosensor ; Bovine serum albumin ; Trypsin ; Scanning photoelectron microscopy
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 April 2016
- 年：2016
- 卷：78
- 期：Complete
- 页码：213-220
- 全文大小：4583 K
文摘
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We report a liquid crystal (LC)sensor system for trypsin detection.
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Trypsin hydrolyzed the surface-immobilized BSA and caused the reorientation of LC.
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The limit of detection of this system for trypsin was 10 ng/mL with specificity.
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The hydrolysis on surfaces was visualized by the scanning photoelectron microscopy.
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This system provides a simple method for fast-screening trypsin activity.