A study on mutual interaction between cytokine induced killer cells and umbilical cord-derived mesenchymal cells: Implication for their in-vivo use
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- 作者：Katia Chieregato ; Elena Albiero ; Silvia Castegnaro ; Martina Bernardi ; Emanuele S.G. d'Amore ; Cristina Zanon ; Domenico Madeo ; Francesco Rodeghiero ; Giuseppe Astori
- 关键词：AD ; adipogenic ; ALPL ; alkaline phosphatase ; BM ; bone marrow ; CIK ; cytokine-induced-killer ; GvHD ; graft-versus-host disease ; GvL ; graft-versus-leukemia ; HSC ; hematopoietic stem cells ; ISCT ; International Society for Cellular Therapy ; MSC ; mesenchymal strom
- 刊名：Blood Cells, Molecules, and Diseases
- 出版年：2012
- 出版时间：15 October-15 December, 2012
- 年：2012
- 卷：49
- 期：3-4
- 页码：159-165
- 全文大小：981 K
文摘
Recently a number of cellular therapy based-clinical trials have been carried out using mesenchymal stromal cells (MSC) or cytokine-induced-killer (CIK) cells aiming to improve outcome of allogeneic hematopoietic stem cell transplantation.
We have isolated MSC from umbilical cord (UC) exploring the interaction between CIK cells and UC-MSC. We found that UC-MSC could suppress CIK cells activity, when co-cultured in a cell-to-cell system. In addition, CIK cells could potentially lyse UC-MSC in a time and ratio dependent manner that could have implications for their in vivo use.
Here we provide experimental data on the mutual interaction of CIK cells and UC-MSC, suggesting a negative interference when the two cell types are used in combination. In the light of our observations, when CIK and UC-MSC will be used in clinical trials, timing and sequencing of their infusion should be considered.