Induction of deletion mutations by methoxyacetaldehyde in Chinese hamster ovary (CHO)-AS52 cells

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• 作者：Chiewchanwit ; Treetip ; Ma ; Hongbao ; El Zein ; Randa ; Hallberg ; Lance ; Au ; William W.
• 关键词：Methoxyacetaldehyde ; Deletion mutation ; Chinese hamster ovary cells ; Polymerase chain reaction
• 刊名：Mutation Research/Environmental Mutagenesis and Related Subjects
• 出版年：1995
• 出版时间：October, 1995
• 年：1995
• 卷：335
• 期：2
• 页码：121-128
• 全文大小：616 K

文摘

We have report previously that methoxyacetaldehyde (MALD), a metabolite of 2-methoxyethanol, induces gpt gene mutations in Chinese hamster ovary (CHO)-AS52 cells but not hprt gene mutations in the standard CHO-K1-BH4 cells. In addition, MALD induces chromosome aberrations in both CHO cell lines. The data presented suggest that MALD induces deletion-type mutations. In this study, we analyzed MALD-induced CHO-AS52 mutants for deletion-type mutations using the nested-polymerase chain reaction (nested-PCR) assay. Spontaneous CHO-AS52 mutants are used as untreated control. Ethylnitrosourea (ENU)-induced CHO-AS52 mutants are used as negative control for multilocus deletions since ENU is a potent inducer of point mutations. The results show that the frequency of MALD-induced mutants containing total deletion of the gpt gene is 42.4 % which is 2.3-fold higher than that from spontaneous mutants (18.6 % ). The frequency of ENU-induced deletion mutation is 3 % . The data substantiate our hypothesis that MALD induces major deletion mutations.