In vivo Antithrombotic and Antiplatelet Activities of a Quantified Acanthopanax sessiliflorus Fruit Extract

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• 作者：Yang SONG^a ; Chun-Juan YANG^a ; Kai YU^a ; Fa-Mei LI ; ^a ; ^{lifamei@syphu.edu.cn"" rel=""nofollow}
• 关键词：Acanthopanax sessiliflorus fruits ; In vivo antithrombotic activity ; Antiplatelet activity ; Shunt thrombosis model ; Triterpenoid ; UPLC/MS/MS
• 刊名：Chinese Journal of Natural Medicines
• 出版年：2011
• 出版时间：March 2011
• 年：2011
• 卷：9
• 期：2
• 页码：141-145
• 全文大小：1252 K

文摘

Aim

To investigate the in vivo antithrombotic and antiplatelet activities and triterpenoid saponin contents of ethanol extract of Acanthopanax sessiliflorus fruits (ASF_EtOH).

Methods

In the present work a quantified ASF_EtOH was selected to determine its in vivo antithrombotic activity in a rat arteriovenous shunt thrombosis model and its inhibitory effect on adenosine diphosphate (ADP)-induced platelet aggregation using a platelet aggregometer. Ten triterpenoid saponin contents of the extract of the fruits were determined by a validated ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method. Total triterpenoid saponin content was also determined according to the colorimetric method by ultraviolet-visible spectrophotometer.

Results

After ASF_EtOH (125, 250, 500 and 1 000 mg·kg⁻¹/d) was orally administered to rats for 15 d, the in vivo antithrombotic activity on rats was in a concentration-dependant manner showing a significant linear relationship for ASF_EtOH (P < 0.05). Meanwhile, ASF_EtOH significantly (P < 0.05) inhibited ADP-induced platelet aggregation in vivo on rats. The inhibitions of (16.3 ± 4.7) % , (28.6 ± 4.1) % , (47.9 ± 4.0) % and (61.3 ± 6.6) % were reached for ASF_EtOH at doses of 125, 250, 500 and 1 000 mg(kg⁻¹ body weight (bw) respectively, compared to an inhibition of (55.4 ± 7.5) % for the positive control aspirin (27 mg(kg⁻¹/d). The contents of the main triterpenoid saponins 22α-hydroxychiisanoside, momordin Ib and chiisanogenin were (3 755 ± 51.3), (2 461 ± 73.0) and (1 114 ± 75.4) μg·g⁻¹ dry fruit respectively. The amount of total triterpenoid saponins in ASF_EtOH was (30.6 ± 0.6) mg of ginsenoside Re equivalents (GRE)/g.

Conclusion

These experimental results suggest that ASF_EtOH has both in vivo antithrombotic and antiplatelet activities.