Generation of Robust Left-Right Asymmetry in the Mouse Embryo Requires a Self-Enhancement and Lateral-Inhibition System

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• 作者：Tetsuya Nakamura ; Naoki Mine ; Etsushi Nakaguchi ; Atsushi Mochizuki ; Masamichi Yamamoto ; Kenta Yashiro ; Chikara Meno ; Hiroshi Hamada
• 关键词：CELLCYCLE
• 刊名：Developmental Cell
• 出版年：2006
• 出版时间：October 2006
• 年：2006
• 卷：11
• 期：4
• 页码：495-504
• 全文大小：678 K

文摘

The bilateral symmetry of the mouse embryo is broken by leftward fluid flow in the node. However, it is unclear how this directional flow is then translated into the robust, left side-specific Nodal gene expression that determines and coordinates left-right situs throughout the embryo. While manipulating Nodal and Lefty gene expression, we have observed phenomena that are indicative of the involvement of a self-enhancement and lateral-inhibition (SELI) system. We constructed a mathematical SELI model that not only simulates, but also predicts, experimental data. As predicted by the model, Nodal expression initiates even on the right side. These results indicate that directional flow represents an initial small difference between the left and right sides of the embryo, but is insufficient to determine embryonic situs. Nodal and Lefty are deployed as a SELI system required to amplify this initial bias and convert it into robust asymmetry.