Analysis of the miRNA expression profile in an Aedes albopictus cell line in response to bluetongue virus infection
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- 作者：Shanshan Xinga ; Junzheng Dua ; dujunzheng@caas.cn" class="auth_mail" title="E-mail the corresponding author ; Shandian Gaoa ; Zhancheng Tiana ; Yadong Zhenga ; Guangyuan Liua ; Jianxun Luoa ; Hong Yina ; b ; yinhong@caas.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：BTV ; bluetongue virus ; CHIKV ; Chikungunya virus ; CPE ; cytopathic effect ; DENV ; dengue fever virus ; dsRNA ; double-stranded RNA ; DMEM ; Dulbecco's modified Eagle's medium ; FBS ; fetal bovine serum ; GO ; gene ontology ; KEGG ; Kyoto Encyclopedia of Genes Genomes ; KOBAS ; KEGG Orthology Based Annotation System ; miRNA ; microRNA ; MOI ; multiplicity of infection ; PBS ; phosphate-buffered saline ; PFU ; plaque-forming units ; piRNA ; PIWI-interacting RNA ; qRT-PCR ; quantitative reverse transcription-polymerase chain
- 刊名：Infection, Genetics and Evolution
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：39
- 期：Complete
- 页码：74-84
- 全文大小：1307 K
文摘
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We evaluated miRNA expression in A. albopictus cells following BTV infection.
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One hundred and forty differentially expressed miRNAs were identified.
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The expression profiles of 11 selected miRNA–mRNA pairs were validated by qRT-PCR.
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The differentially expressed miRNAs are likely to be regulators of BTV infection.
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This is the first report on the effect of BTV infection on miRNA expression.