Evidence for natural vertical transmission of chikungunya viruses in field populations of Aedes aegypti in Delhi and Haryana states in India—a preliminary report

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• 作者：Jaspreet Jain^a ; ^{jaspreet.jain@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Raja Babu S. Kushwah^a ; ^b ; ^{kushwah001@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Shashi S. Singh^a ; ¹ ; ^{shekharsbtjnu@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Anil Sharma^a ; ² ; ^{anil.mrc@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Tridibes Adak^b ; ^{adak.mrc@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Om P. Singh^b ; ^{singh@mrcindia.org" class="auth_mail" title="E-mail the corresponding author} ; Raj Kamal Bhatnagar^a ; ^{raj@icgeb.res.in" class="auth_mail" title="E-mail the corresponding author} ; Sarala K. Subbarao^c ; ^{subbaraosk@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Sujatha Sunil^a ; ^{sujatha@icgeb.res.in" class="auth_mail" title="E-mail the corresponding author}
• 关键词：A. aegypti ; Chikungunya ; Minimum infection rate (MIR) ; Vertical transmission
• 刊名：Acta Tropica
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：162
• 期：Complete
• 页码：46-55
• 全文大小：1930 K

文摘

Aedes aegypti and Aedes albopictus are principal vectors for the transmission of chikungunya virus (CHIKV). India is a hub for both dengue and chikungunya infections and there are several reports of co-infection of dengue and chikungunya virus in the clinical scenario. The present pilot entomological survey was conducted to evaluate vertical transmission of CHIKV in Aedes field populations. Aedes immature (larvae and pupae) collection was done in 2012, over a period of six months from selected sites in Delhi and Haryana, India. The immatures collected were reared for adult emergence and species identification was done. A. aegypti male and female mosquitoes were separated and pooled collection spot-wise, RNA extracted and RT PCR performed to test for the presence of CHIKV in the pools. Container index (CI) and minimum infection rate (MIR) were estimated. From study areas that tested positive for CHIKV, adult collections were made and females upon feeding on uninfected blood in laboratory were allowed to lay eggs. The progeny that emerged from these field-collected mothers were tested for CHIKV presence. Our pilot survey showed the existence of A. aegypti population even during peak summer season in a few foci which eventually helped the mosquitoes to tide over adverse environmental conditions and with the start of rainfall, the population exploded within a short period of time. Immatures collected from field and progeny of adults collected from the field were CHIKV positive demonstrating the presence of vertical transmission of chikungunya virus in field population of A. aegypti. The present study further demonstrates the importance of identifying permanent breeding sites for proper Aedes species control.