Microarray analyses for identifying genes conferring resistance to pepper leaf curl virus in chilli pepper (Capsicum spp.)

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• 作者：Ved Prakash Rai^a ; ^b ; ¹ ; Ashutosh Rai^b ; Rajesh Kumar^b ; ^{rajes74@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Sanjay Kumar^c ; Sanjeet Kumar^d ; Major Singh^b ; Sheo Pratap Singh^a
• 关键词：Begomovirus ; Molecular mechanism ; Cross species hybridization ; Agilent
• 刊名：Genomics Data
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：9
• 期：Complete
• 页码：140-142
• 全文大小：267 K

文摘

Pepper leaf curl virus (PepLCV) is a serious threat to pepper (Capsicum spp.) production worldwide. Molecular mechanism underlying pepper plants response to PepLCV infection is key to develop PepLCV resistant varieties. In this study, we generated transcriptome profiles of PepLCV resistant genotype (BS-35) and susceptible genotype (IVPBC-535) after artificial viral inoculation using microarray technology and detail experimental procedures and analyses are described. A total of 319 genes differentially expressed between resistant and susceptible genotypes were identified, out of that 234 unique genes were found to be up-regulated > 2-fold in resistant line BS-35 when compared to susceptible, IVPBC-535. The data set we generated has been analyzed to identify genes that are involved in the regulation of resistance against PepLCV. The raw data have been deposited in the Gene Expression Omnibus (GEO) database under accession number GSE41131.