The three tested biological indicators do not provide consistent images of global ecosystem quality. QuiroRius is useful as a biological indicator providing complementary information on riparian forest quality but cannot be used alone to fully evaluate riparian ecosystem health. Wide rivers with well-structured native riparian forests are the best habitat for females. Landscape composition at larger scales and greater altitudes should be neglected when analysing the usefulness of Myotis daubentonii as a biological indicator below 1000 m a.s.l.