Characterisation and developmental expression of a chitinase gene in Heterodera glycines
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- 作者：Gao ; Bingli ; Allen ; R. ; Maier ; Tom ; McDermott ; Jeff P. ; Davis ; Eric L. ; Baum ; Thomas J. ; Hussey ; Richard S.
- 关键词：Chitinase ; Oesophageal gland cells ; Gene sequence ; Nematoda ; Plant-parasitic nematode
- 刊名：International Journal for Parasitology
- 出版年：2002
- 出版时间：September, 2002
- 年：2002
- 卷：32
- 期：10
- 页码：1293-1300
- 全文大小：507 K
文摘
A chitinase full-length cDNA (designated Hg-chi-1) was isolated from a Heterodera glycines oesophageal gland cell-specific long-distance PCR cDNA library. The cDNA hybridised to genomic DNA of H. glycines in Southern blots. The Hg-chi-1 cDNA contained an open reading frame encoding 350 amino acids with the first 23 amino acids being a putative signal peptide for secretion. Hg-CHI-1 contained a chitinase 18 family catalytic domain, and chitinolytic activity of recombinant Hg-CHI-1 was confirmed in glycol–chitin substrate gel electrophoresis. In situ mRNA hybridisation analyses showed that transcripts of Hg-chi-1 accumulated specifically in the subventral oesophageal gland cells of parasitic stages of H. glycines, but Hg-chi-1 expression was not detected in eggs or hatched pre-parasitic second-stage juveniles, suggesting that this chitinase does not have a role in egg hatching of H. glycines. The biological function of Hg-CHI-1 in H. glycines remains to be determined.