Surface modified alginate microcapsules for 3D cell culture
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- 作者：Yi-Wen Chen ; Chiung Wen Kuo ; kuo55@gate.sinica.edu.tw" class="auth_mail" title="E-mail the corresponding author ; Di-Yen Chueh ; Peilin Chen ; peilin@gate.sinica.edu.tw" class="auth_mail" title="E-mail the corresponding author
- 关键词：Coating ; Non fouling surface ; Microcapsules ; 3D culture
- 刊名：Surface Science
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：648
- 期：Complete
- 页码：47-52
- 全文大小：650 K
文摘
Culture as three dimensional cell aggregates or spheroids can offer an ideal platform for tissue engineering applications and for pharmaceutical screening. Such 3D culture models, however, may suffer from the problems such as immune response and ineffective and cumbersome culture. This paper describes a simple method for producing microcapsules with alginate cores and a thin shell of poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) to encapsulate mouse induced pluripotent stem (miPS) cells, generating a non-fouling surface as an effective immunoisolation barrier. We demonstrated the trapping of the alginate microcapsules in a microwell array for the continuous observation and culture of a large number of encapsulated miPS cells in parallel. miPS cells cultured in the microcapsules survived well and proliferated to form a single cell aggregate. Droplet formation of monodisperse microcapsules with controlled size combined with flow cytometry provided an efficient way to quantitatively analyze the growth of encapsulated cells in a high-throughput manner. The simple and cost-effective coating technique employed to produce the core–shell microcapsules could be used in the emerging field of cell therapy. The microwell array would provide a convenient, user friendly and high-throughput platform for long-term cell culture and monitoring.