A primer and probe set for detecting multiple types of EGFR exon 19 deletions
详细信息 查看全文
- 作者：Tai-Long Chena ; 1 ; John Wen-Cheng Changb ; c ; 1 ; Chih-Liang Wangd ; Cheng-Ta Yangd ; e ; Mei-Chia Wanga ; f ; Chiuan-Chian Chioua ; d ; g ; ccchiou@mail.cgu.edu.tw" class="auth_mail" title="E-mail the corresponding author
- 关键词：EGFR mutation ; Peptide nucleic acid clamp ; Fluorescent probe ; Forster resonance energy transfer ; Melting analysis
- 刊名：Analytical Biochemistry
- 出版年：2016
- 出版时间：15 November 2016
- 年：2016
- 卷：513
- 期：Complete
- 页码：61-67
- 全文大小：1261 K
文摘
- •
A homogeneous assay detects at least 29 types of EGFR exon 19 deletions using a simple primer and probe set.
- •
Fluorescent labeled probe pair generates mutation signals through Foster resonance energy transfer in a melting analysis.
- •
A peptide nucleic acid clamp allows amplification and detection of 0.01% mutant DNA in wild-type background.
- •
Mutations can be detected in pleural effusions and plasma from lung cancer patients.