Significance of sulfiredoxin/peroxiredoxin and mitochondrial respiratory chain in response to and protection from 100 % O₂ in Saccharomyces cerevisiae

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• 作者：Hiroki Fujiwara ; Shigeyuki Kawai ; Kousaku Murata ; ^{kmurata@kais.kyoto-u.ac.jp}
• 关键词：ROS ; reactive oxygen species ; SOD ; superoxide dismutase ; Prx ; peroxiredoxin ; WT ; wild-type ; qPCR ; quantitative PCR ; CCCP ; carbonyl cyanide m-chlorophenyl hydrazone ; DMSO ; dimethyl sulfoxide ; SC ; synthetic complete ; YPD ; yeast extract/peptone/dextrose ; DIC
• 刊名：Mitochondrion
• 出版年：2013
• 出版时间：January, 2013
• 年：2013
• 卷：13
• 期：1
• 页码：52-58
• 全文大小：650 K

文摘

The mechanisms underlying organisms respond to and protect from hyperoxia remain elusive. We establish a system for cultivating the yeast m>Saccharomyces cerevisiaem> cells in liquid medium under 100 % O₂ and revealed that m>SRX1m>, encoding sulfiredoxin, is significantly induced by 100 % O₂ dependently on transcription factors Yap1 and Skn7. Sulfiredoxin has a role in restoring the abundant peroxiredoxin, Tsa1. Tsa1 was indispensable for protection from 100 % O₂ in the presence of antimycin A, an inhibitor of complex III in the mitochondrial respiratory chain, collectively emphasizing the significance of sulfiredoxin, peroxiredoxin, and mitochondrial respiratory chain to respond to and to protect from 100 % O₂.