Proteomics of a new esophageal cancer cell line established from Persian patient

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• 作者：Mehdi Moghanibashi^a ; ^{mehdimoghani@yahoo.com} ; Ferdous Rastgar Jazii^b ; ^c ; ^{rastgarjazii@googlemail.com} ; Zahra-Soheila Soheili^b ; Maryam Zare^b ; ^d ; Aliasghar Karkhane^b ; Kazem Parivar^a ; Parisa Mohamadynejad^e
• 关键词：ESCC ; esophageal squamous cell carcinoma ; 2DE ; two dimensional gel electrophoresis ; DMEM/F12 ; Dulbecco's modified Eagle's F12 ; EGF ; epidermal growth factor ; BPE ; bovine pituitary extract ; CHAPS ; cholamidopropyl dimethyl ammonio propane sulfonate ; DTT ; dit
• 刊名：Gene
• 出版年：2012
• 出版时间：25 May, 2012
• 年：2012
• 卷：500
• 期：1
• 页码：124-133
• 全文大小：1479 K

文摘

Although the highest incidence of esophageal squamous cell carcinoma (ESCC) has repeatedly been reported from Persia (Iran), nevertheless the so far proteomic published reports were limited to one study on tissue specimens. Here we report the proteome of a newly established cell line from Persian ESCC patients and compare it with the normal primary cell proteome. Among polypeptides, whose expression was different in cell line sixteen polypeptides were identified by MALDI/TOF/TOF spectrometry. S100-A8 protein, annexin A1, annexin A2, regulatory subunit of calpain, subunit alpha type-3 of proteasome and glutamate dehydrogenase 1 were proteins down-regulated in cell line while peroxiredoxin-5, non-muscle myosin light polypeptide 6, keratin 1, annexin A4, keratin 8, tropomyosin 3, stress-induced-phosphoprotein 1 and albumin were found to be subject of up-regulation in cell line compared to the primary normal cells. The proteomic results were further verified by western blotting and RT-PCR on annexin A1 and keratin 8. In addition, among the aforementioned proteins, glutamate dehydrogenase 1, regulatory subunit of calpain, subunit alpha of type-3 proteasome and annexin A4 are proteins whose deregulation in ESCC is reported for the first time by this study.