Monoclonal antibody to the δ opioid receptor acts as an agonist in dual regulation of adenylate cyclase in NG108-15 cells

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• 作者：Gomes ; Ivone ; Gupta ; Achla ; Singh ; S.P. ; Sharma ; Shail K.
• 关键词：Adenylate cyclase ; Antibody ; cAMP ; G protein ; Opioid receptor ; Signal transduction ; cAMP ; cyclic adenosine-3&prime ; 5&prime ; -monophosphate ; CHAPS ; 3-[(3-cholamidopropyl) dimethyl ammonio] 1-propane-sulfonate ; DAMGO ; [D-Ala² ; N-methyl-Phe^4<
• 刊名：FEBS Letters
• 出版年：1999
• 出版时间：July 30, 1999
• 年：1999
• 卷：456
• 期：1
• 页码：126-130
• 全文大小：170 K

文摘

Monoclonal antibodies generated against multiple antigenic peptides of the N-terminal sequence (³LVPSARAELQSSPLV¹⁷) of the cloned δ opioid receptor immunoprecipitated a 58 kDa protein from CHAPS-solubilized NG108-15 membranes. The immunoprecipitates bound [³H]DPDPE – but not [³H]DAMGO – with a K_d of 6.4 nM and a B_max of 75 pM. Western blot analysis revealed a distinct band of 58 kDa. The antibodies inhibited basal and PGE₁-stimulated cAMP levels, and mimicked the effect of agonists manifest in a compensatory increase in cAMP formation. The antibody will be potentially useful in the analysis of functional epitopes on the δ opioid receptor.