Regulation of anti-LDL immobilization on self-assembled protein G layer using CHAPS and its application to immunosensor

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• 作者：Choi ; Jeong-Woo ; Park ; Ki-Suk ; Lee ; Woochang ; Oh ; Byung-Keun ; Chun ; Bum-Suk ; Paek ; Se-Hwan
• 关键词：Self-assembled monolayer ; CHAPS ; Protein G ; Anti-LDL ; Immunosensor
• 刊名：Materials Science and Engineering: C
• 出版年：2004
• 出版时间：January 5, 2004
• 年：2004
• 卷：24
• 期：1-2
• 页码：241-245
• 全文大小：1.04 M

文摘

In antibody-based immunoassay, it is important to fabricate a structured base plate with high activity for antibody immobilization. To prevent the reduction of anti-LDL binding capacity due to protein G aggregation, detergent was introduced to control the size of protein G aggregate. The self-assembled monolayer of 11-mercaptoundecanoic acid (11-(MUA)) and hexanethiol mixture was fabricated to form the stable protein G layer. 3-[(cholamidopropyl)dimethyl-ammonio]-1-propane sulfonate (CHAPS) was used for the regulation of protein G aggregate immobilized on the 11-(MUA) surface. The effect of various CHAPS concentrations on the amount of anti-low density lipoprotein (LDL) immobilized on protein G layer was investigated. Twelve millimolars of CHAPS were determined as the optimal concentration to maximize the binding capacity of anti-LDL due to the control of protein G density on the surface. The anti-LDL layer on self-assembled protein G using CHAPS was applied to surface plasmon resonance immunosensor for detection of LDL and its detection limit was 100 pM.